The isolation and purification of human peripheral blood monocytes in cell suspension. Academic Article uri icon

Overview

abstract

  • A simple method for the isolation of human peripheral blood monocytes in cell suspension is described. The method is based on the adherence and detachment characteristics of monocytes during their in vitro cultivation and does not require any gradient centrifugation or modulating substances. The mean percentage of monocytes recovered from different donors was 81.9% (60-100%) of the original phagocytic cells found in the peripheral blood of the tested donors and the cell suspensions obtained consisted of more than 95% of monocytes. The monocytes isolated in suspensions show high activity in various monocyte functions tested.

publication date

  • January 1, 1980

Research

keywords

  • Cell Separation
  • Monocytes

Identity

Scopus Document Identifier

  • 0019163809

Digital Object Identifier (DOI)

  • 10.1016/0022-1759(80)90295-1

PubMed ID

  • 7462647

Additional Document Info

volume

  • 39

issue

  • 1-2