Alpha-1 subunits of voltage gated Ca2+ channels in the mesencephalon x neuroblastoma hybrid cell line MES23.5. Academic Article uri icon

Overview

abstract

  • The identity of alpha 1 subunits from voltage operated Ca2+ channels was determined in the rat/mouse mesencephalon x N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(alpha 1D), Q-(alpha 1A) and omega-conotoxin GVIA sensitive N-type (alpha 1B) Ca2+ channel alpha 1 subunits. The amplified fragments, which are homologous to the region between domain III and IV of known alpha 1 subunits, reveal splice variation in the L- and Q-type alpha 1 subunit of MES23.5 cells. The transcripts of alpha 1 subunits in these cells were quantified by RNAase protection assay. The data show the existence of different Ca2+ channel types in a single cell line and may reflect multiple functions of voltage operated Ca2+ channels during growth, differentiation and transmitter release.

publication date

  • September 1, 1995

Research

keywords

  • Brain Neoplasms
  • Calcium Channels
  • Ion Channel Gating
  • Mesencephalon
  • Neuroblastoma

Identity

Scopus Document Identifier

  • 0029015029

Digital Object Identifier (DOI)

  • 10.1016/0306-4522(95)00147-b

PubMed ID

  • 7477958

Additional Document Info

volume

  • 68

issue

  • 2