Endometrial secretory proteins enhance early embryo development. Academic Article uri icon

Overview

abstract

  • PURPOSE: To evaluate the role of endometrial stromal cells and their secretory proteins in early embryo development, two-celled CB6F1 mouse embryos were cultured alone or cocultured with human endometrial stromal cells in various culture conditions. RESULTS: The percentage of embryo blastocyst formation, hatching, and outgrowth was significantly greater in (1) coculture with endometrial stromal cells than in a cell-free control when both coculture and control were carried out in protein-free medium or in RPMI 1640 plus 10% fetal calf serum; (2) coculture with hormone (i.e., progesterone plus relaxin)-treated cells than in coculture with hormone-nontreated cells; and (3) media supplemented with isolated endometrial secretory proteins than in media supplemented with BSA (0.35%). Embryo development was not found to be significantly different in coculture and in media supplemented with endometrial secretory protein. CONCLUSION: Our data provides credence to the theory that endometrial stromal cells enhance embryo development by secreting specific proteins that are beneficial to embryo growth in vitro.

publication date

  • April 1, 1994

Research

keywords

  • Embryonic and Fetal Development
  • Endometrium
  • Proteins

Identity

Scopus Document Identifier

  • 0028603263

Digital Object Identifier (DOI)

  • 10.1007/BF02211811

PubMed ID

  • 7711384

Additional Document Info

volume

  • 11

issue

  • 4