Use of oligonucleotide probes to analyse the homology of the oprF gene among clinical and heterologous immunotype strains of Pseudomonas aeruginosa. Academic Article uri icon

Overview

abstract

  • The conservation of oprF gene among 25 clinical Pseudomonas aeruginosa strains and a set of 17 serotype-specific representative strains of the international antigen typing scheme (IATS) was analysed by dot-blotting using five specific oligonucleotide probes. The oligo 1, 2, 3, 4, 5 correspond to five different regions of the oprF gene and hybridized strongly with respectively 88%, 88%, 76%, 94% and 71% of the IATS strains and 88%, 96%, 92%, 88% and 92% of the clinical strains. A parallel study performed with the whole oprF gene showed a lack of specificity of this probe: indeed, the probe hybridized not only with the 42 Pseudomonas aeruginosa strains but also with Escherichia coli and Salmonella minnesota. This study suggests that the gene sequence encoding the protein F is not totally conserved among Pseudomonas aeruginosa strains.

publication date

  • October 1, 1994

Research

keywords

  • Oligonucleotide Probes
  • Porins
  • Pseudomonas aeruginosa

Identity

Scopus Document Identifier

  • 0028037463

Digital Object Identifier (DOI)

  • 10.1006/mcpr.1994.1056

PubMed ID

  • 7877635

Additional Document Info

volume

  • 8

issue

  • 5