The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. Academic Article uri icon

Overview

abstract

  • The S. cerevisiae VPS10 (vacuolar protein sorting) gene encodes a type I transmembrane protein of 1577 amino acids required for the sorting of the soluble vacuolar protein carboxypeptidase Y (CPY). Mutations in VPS10 result in the selective missorting and secretion of CPY; all other vacuolar proteins tested are delivered to the vacuole in vps10 mutants. Chemical cross-linking studies demonstrate that Vps10p and the Golgi-modified precursor form of CPY directly interact. A single amino acid change in the CPY vacuolar sorting signal prevents this interaction. Vps10p also interacts with a hybrid protein containing the CPY sorting signal fused to the normally secreted enzyme invertase. Subcellular fractionation indicates that the majority of Vps10p is localized to a late Golgi compartment where vacuolar proteins are sorted. We propose that VPS10 encodes a CPY sorting receptor that executes multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment.

publication date

  • May 20, 1994

Research

keywords

  • Carboxypeptidases
  • Fungal Proteins
  • Genes, Fungal
  • Membrane Proteins
  • Receptors, Cell Surface
  • Receptors, Cytoplasmic and Nuclear
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Vacuoles
  • Vesicular Transport Proteins

Identity

Scopus Document Identifier

  • 0028332917

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(94)90219-4

PubMed ID

  • 8187177

Additional Document Info

volume

  • 77

issue

  • 4