Catecholamine histofluorescence using cryostat sectioning and glyoxylic acid in unperfused frozen brain: a detailed description of the technique. Academic Article uri icon

Overview

abstract

  • This paper presents a technique for glyoxylic acid-induced monoamine histofluorescence in the central nervous system. Unperfused rat brains are sectioned in a cryostat, immersed in 2% glyoxylic acid solution, warm-air dried and gassed at 100 degrees C. Intense, well-localized catecholamine fluorescence is produced and all known catecholamine-containing structures are demonstrated. The fluorescence obtained by this method was evaluated by a variety of agents and was shown to be catecholaminergic in origin. In contrast to the Vibratome-glyoxylic acid technique, this procedure reliably produces thin, whole-brain sections of even thickness and allows protracted use of the tissue block. Because unperfused tissue is used, the technique can be applied to a broad variety of material, such as post-mortem tissue or invertebrate preparations. Alternate sections can be prepared for a variety of techniques requiring unperfused tissue (e.g., enzymatic localization, chemical assay, anatomical techniques). The reasons for choosing each of the parameters in the technique are discussed.

publication date

  • March 1, 1977

Research

keywords

  • Brain Chemistry
  • Dopamine
  • Histocytochemistry
  • Norepinephrine
  • Serotonin

Identity

Scopus Document Identifier

  • 0017337585

Digital Object Identifier (DOI)

  • 10.1007/BF01003630

PubMed ID

  • 838603

Additional Document Info

volume

  • 9

issue

  • 2