A monoclonal antibody to the CDR-3 region of CD4 inhibits soluble CD4 binding to virions of human immunodeficiency virus type 1. Academic Article uri icon

Overview

abstract

  • The CDR-3 region of CD4 has been proposed to be involved in the fusion reaction between human immunodeficiency virus type 1 (HIV-1) and CD4+ cells, either at a stage involving virus binding or subsequent to virus binding. Part of the evidence for this has been the observation that monoclonal antibodies (MAbs) to CDR-3 block HIV infection potently without strongly inhibiting the binding of monomeric gp120 to CD4. Here I show that, in a system using oligomeric, virion-bound gp120, a MAb to CDR-3 resembles those to CDR-2 in that it inhibits soluble CD4 binding to virions. Consequently, ternary complexes of MAb-soluble CD4-gp120 cannot be detected with CDR-2 MAbs and are detectable only at a very low level with a CDR-3 MAb, but they clearly form when a control MAb to CD4 domain 4 is used. Although not in direct conflict with previously published data on the role of CDR-3 MAbs in the inhibition of HIV-1 infection, these experiments do not support the hypothesis that the CDR-3 region is specifically involved in virus entry at a postbinding stage.

publication date

  • June 1, 1993

Research

keywords

  • Acquired Immunodeficiency Syndrome
  • CD4 Antigens
  • HIV-1
  • Virion

Identity

PubMed Central ID

  • PMC237720

Scopus Document Identifier

  • 0027278133

Digital Object Identifier (DOI)

  • 10.1128/JVI.67.6.3656-3659.1993

PubMed ID

  • 8497074

Additional Document Info

volume

  • 67

issue

  • 6