Synthetic peptides entrapped in microparticles can elicit cytotoxic T cell activity. Academic Article uri icon

Overview

abstract

  • Peptides from Plasmodium berghei circumsporozoite protein (CS) and influenza A virus nucleoprotein (NP) were entrapped in microparticles prepared from poly (lactide-co-glycolide) polymers, and the microparticles were administered parenterally to mice. After immunization with single or multiple doses, splenocytes were tested for a cytotoxic T cell (CTL) response and high levels of CTL activity were detected. The CTL induced were CD8+, MHC class I restricted, and could recognize virus infected cells. Peptide entrapped in microparticles of mean size < 500 nm were better inducers of CTL than larger microparticles (mean > 2 microns and above). Microparticles could also be used to deliver lipid modified peptides (lipopeptides) and elicited higher levels of cytolytic activity than either free peptide in microparticles or lipopeptide alone. Microparticles provide a novel way of inducing a CTL response using synthetic peptides.

authors

  • Nixon, Douglas
  • Hioe, Catarina
  • Chen, P D
  • Bian, Zuning
  • Kuebler, Peter
  • Li, M L
  • Qiu, Howard
  • Li, X M
  • Singh, Manmohan
  • Richardson, Julie
  • McGee, Paul
  • Zamb, Tim
  • Koff, Wayne
  • Wang, C Y
  • O'Hagan, Derek

publication date

  • November 1, 1996

Research

keywords

  • Antigens, Protozoan
  • Antigens, Viral
  • Lactic Acid
  • Peptides
  • Polyglycolic Acid
  • T-Lymphocytes, Cytotoxic

Identity

Scopus Document Identifier

  • 0001552479

Digital Object Identifier (DOI)

  • 10.1016/s0264-410x(96)00099-0

PubMed ID

  • 9014294

Additional Document Info

volume

  • 14

issue

  • 16