The p53 status of Chinese hamster V79 cells frequently used for studies on DNA damage and DNA repair. Academic Article uri icon

Overview

abstract

  • Chinese hamster lung fibroblast V79 cells have been widely used in studies of DNA damage and DNA repair. Since the p53 gene is involved in normal responses to DNA damage, we have analyzed the molecular genetics and functional status of p53 in V79 cells and primary Chinese hamster embryonic fibroblast (CHEF) cells. The coding product of the p53 gene in CHEF cells was 76 and 75% homologous to human and mouse p53 respectively, and was 95% homologous to the Syrian hamster cells. The V79 p53 sequence contained two point mutations located within a presumed DNA binding domain, as compared with the CHEF cells. Additional immunocytochemical and molecular studies confirmed that the p53 protein in V79 cells was mutated and nonfunctional. Our results indicate that caution should be used in interpreting studies of DNA damage, DNA repair and apoptosis in V79 cells.

publication date

  • March 1, 1997

Research

keywords

  • DNA Damage
  • DNA Repair
  • Tumor Suppressor Protein p53

Identity

PubMed Central ID

  • PMC146528

Digital Object Identifier (DOI)

  • 10.1093/nar/25.5.992

PubMed ID

  • 9023109

Additional Document Info

volume

  • 25

issue

  • 5