Secretory leukocyte protease inhibitor: a macrophage product induced by and antagonistic to bacterial lipopolysaccharide. Academic Article uri icon

Overview

abstract

  • To explore regulation of potentially lethal responses to bacterial lipopolysaccharide (LPS), we used differential display under LPS-free conditions to compare macrophage cell lines from two strains of mice congenic for a locus affecting LPS sensitivity. LPS-hyporesponsive cells, primary macrophages, and polymorphonuclear leukocytes transcribed secretory leukocyte protease inhibitor (SLPI), a known epithelial cell-derived inhibitor of leukocyte serine proteases. Transfection of macrophages with SLPI suppressed LPS-induced activation of NF-kappa B and production of nitric oxide and TNF alpha. The ability of interferon-gamma (IFN gamma) to restore LPS responsiveness is a hallmark of the LPS-hyporesponsive phenotype. IFN gamma suppressed expression of SLPI and restored LPS responsiveness to SLPI-producing cells. Thus, SLPI is an LPS-induced IFN gamma-suppressible phagocyte product that serves to inhibit LPS responses.

publication date

  • February 7, 1997

Research

keywords

  • Lipopolysaccharides
  • Macrophages
  • Protein Biosynthesis
  • Proteins
  • Serine Proteinase Inhibitors

Identity

Scopus Document Identifier

  • 0030941175

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(00)81880-2

PubMed ID

  • 9039268

Additional Document Info

volume

  • 88

issue

  • 3