The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole. Academic Article uri icon

Overview

abstract

  • Three distinct adaptor protein (AP) complexes involved in protein trafficking have been identified. AP-1 and AP-2 mediate protein sorting at the trans-Golgi network and plasma membrane, respectively, whereas the function of AP-3 has not been defined. A screen for factors specifically involved in transport of alkaline phosphatase (ALP) from the Golgi to the vacuole/lysosome has identified Ap16p and Ap15p of the yeast AP-3 complex. Deletion of each of the four AP-3 subunits results in selective mislocalization of ALP and the vacuolar t-SNARE, Vam3p (but not CPS and CPY), while deletion of AP-1 and AP-2 subunits has no effect on vacuolar protein delivery. This study, therefore, provides evidence that the AP-3 complex functions in cargo-selective protein transport from the Golgi to the vacuole/lysosome.

publication date

  • October 3, 1997

Research

keywords

  • Alkaline Phosphatase
  • Fungal Proteins
  • Monomeric Clathrin Assembly Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Vacuoles

Identity

Scopus Document Identifier

  • 0030886261

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(01)80013-1

PubMed ID

  • 9335339

Additional Document Info

volume

  • 91

issue

  • 1