Generation or large numbers of immature and mature dendritic cells from rat bone marrow cultures. Academic Article uri icon

Overview

abstract

  • We have defined conditions for generating large numbers of dendritic cells (DC) in marrow cultures from 10-12-week-old ACI or WF rats. The combination of granulocyte-macrophage colony-stimulating factor (GM-CSF) and TNF-alpha, known to induce DC from human CD34+ progenitors, was not effective with rat. In contrast, GM-CSF plus IL-4 generated DC in high yield, corresponding to 30-40% of the initial number of plated marrow cells. The DC proliferated in distinctive aggregates, in which most cells had an immature phenotype marked by undetectable surface B7 and high levels of MHC class II products within intracellular lysosomes. When dislodged and dispersed, the aggregates gave rise to mature stellate DC with abundant surface MHC class II and B7, sparse MHC class II- lysosomes, and strong T cell-stimulating capacity. Therefore, rat marrow progenitors can generate large numbers of immature DC, with abundant intracellular MHC class II compartments, and potent, stimulatory, mature DC.

publication date

  • March 1, 1998

Research

keywords

  • Bone Marrow Cells
  • Dendritic Cells

Identity

Scopus Document Identifier

  • 0031881534

Digital Object Identifier (DOI)

  • 10.1002/(SICI)1521-4141(199803)28:03<811::AID-IMMU811>3.0.CO;2-S

PubMed ID

  • 9541575

Additional Document Info

volume

  • 28

issue

  • 3