Increased VH 11 and VH Q52 gene use by splenic B cells in old mice associated with oligoclonal expansions of CD5 + B cells. Academic Article uri icon

Overview

abstract

  • A significant increase in the utilization of the VH gene families VH11 and Q52 was observed in LPS-stimulated splenic B lymphocytes from aged mice compared to young mice. VH gene usage was assayed by in situ DNA/RNA hybridization using VH family-specific and kappa chain probes. The observed age-dependent differences appear to reflect the preferential use of VH11 and Q52 VH gene use by the CD5 + B lymphocyte subset whose numbers in the spleen increase with age. The increased use of VH11 by splenic cells from old mice is associated with clonal expansions of splenic CD5 + B lymphocytes.

publication date

  • June 15, 1998

Research

keywords

  • Aging
  • B-Lymphocyte Subsets
  • B-Lymphocytes
  • Immunoglobulin Heavy Chains

Identity

Scopus Document Identifier

  • 0032525348

Digital Object Identifier (DOI)

  • 10.1016/s0047-6374(98)00004-9

PubMed ID

  • 9701765

Additional Document Info

volume

  • 103

issue

  • 2