Development of a flexible and specific gene delivery system for production of murine tumor models. Review uri icon

Overview

abstract

  • To develop models of human cancer we have expressed the avian retroviral receptor, TVA, under a variety of mammalian promoters in transgenic mice, thus rendering mice susceptible to infection with avian leukosis virus-derived gene vectors. TVA-based retroviral gene transfer offers advantages over current murine models of human cancer. A single transgenic mouse line can be used to evaluate multiple genetic lesions, individually and in combination. Furthermore, mutant genes are introduced somatically into animals, as occurs in the majority of naturally occurring tumors. Because the avian viral vectors replicate only in avian cells, the viral receptor in infected transgenic mouse cells remains available for multiple rounds of infection with different ASLV vectors. We discuss the theoretical and practical aspects of using recombinant avian retroviruses with TVA transgenic mice to generate cancer models.

publication date

  • September 20, 1999

Research

keywords

  • Disease Models, Animal
  • Gene Transfer Techniques
  • Neoplasms

Identity

Scopus Document Identifier

  • 0033588840

Digital Object Identifier (DOI)

  • 10.1038/sj.onc.1203087

PubMed ID

  • 10498877

Additional Document Info

volume

  • 18

issue

  • 38