Hedgehog-regulated processing of Gli3 produces an anterior/posterior repressor gradient in the developing vertebrate limb. Academic Article uri icon

Overview

abstract

  • Ci/Gli zinc finger proteins mediate the transcriptional effects of Hedgehog protein signals. In Drosophila, Ci action as transcriptional repressor or activator is contingent upon Hedgehog-regulated, PKA-dependent proteolytic processing. We demonstrate that PKA-dependent processing of vertebrate Gli3 in developing limb similarly generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The resulting anterior/posterior Gli3 repressor gradient can be perturbed by mutations of Gli3 in human genetic syndromes or by misregulation of Gli3 processing in the chicken mutant talpid2, producing a range of limb patterning malformations. The high relative abundance and potency of Gli3 repressor suggest specialization of Gli3 and its products for negative Hedgehog pathway regulation.

publication date

  • February 18, 2000

Research

keywords

  • DNA-Binding Proteins
  • Limb Buds
  • Nerve Tissue Proteins
  • Protein Processing, Post-Translational
  • Proteins
  • Repressor Proteins
  • Trans-Activators
  • Transcription Factors
  • Xenopus Proteins

Identity

Scopus Document Identifier

  • 0034681266

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(00)80678-9

PubMed ID

  • 10693759

Additional Document Info

volume

  • 100

issue

  • 4