Src tyrosine kinase is a novel direct effector of G proteins. Academic Article uri icon

Overview

abstract

  • Heterotrimeric G proteins transduce signals from cell surface receptors to modulate the activity of cellular effectors. Src, the product of the first characterized proto-oncogene and the first identified protein tyrosine kinase, plays a critical role in the signal transduction of G protein-coupled receptors. However, the mechanism of biochemical regulation of Src by G proteins is not known. Here we demonstrate that Galphas and Galphai, but neither Galphaq, Galpha12 nor Gbetay, directly stimulate the kinase activity of downregulated c-Src. Galphas and Galphai similarly modulate Hck, another member of Src-family tyrosine kinases. Galphas and Galphai bind to the catalytic domain and change the conformation of Src, leading to increased accessibility of the active site to substrates. These data demonstrate that the Src family tyrosine kinases are direct effectors of G proteins.

publication date

  • September 1, 2000

Research

keywords

  • Heterotrimeric GTP-Binding Proteins
  • src-Family Kinases

Identity

Scopus Document Identifier

  • 0034268796

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(00)00086-6

PubMed ID

  • 11007482

Additional Document Info

volume

  • 102

issue

  • 5