Olive oil decreases both oxidative stress and the production of arachidonic acid metabolites by the prostaglandin G/H synthase pathway in rat macrophages. Academic Article uri icon

Overview

abstract

  • Fish oil has a preventive role in cardiovascular and inflammatory diseases, but little is known about the effect of olive oil, which is widely consumed in Mediterranean regions. We examined the influence of dietary olive oil, corn oil and fish oil-rich diets on the production of superoxide anion (O2-) and nitric oxide (.NO) by resident macrophages stimulated by phorbol 12-myristate 13-acetate (PMA) and their effect on arachidonic acid release, prostaglandin G/H synthase-2 (PGHS-2) expression and the subsequent prostaglandin E(2) production. Resident peritoneal macrophages stimulated by PMA from rats fed with olive oil or corn oil had the same level of O2- production, but these levels were increased by the fish oil diet. Olive oil and the fish oil diets increased .NO and decreased arachidonic acid mobilization and the production of prostaglandin E(2). PGHS-2 expression, however, was not affected by diet. We conclude that although olive oil and fish oil reduce arachidonic acid mobilization and subsequent metabolism through the PGHS-2 pathway in PMA-stimulated macrophages, only olive oil offers an additional beneficial effect by increasing .NO/O2- production.

publication date

  • August 1, 2001

Research

keywords

  • Arachidonic Acid
  • Dinoprostone
  • Macrophages, Peritoneal
  • Oxidative Stress
  • Plant Oils
  • Prostaglandin-Endoperoxide Synthases

Identity

Scopus Document Identifier

  • 0034900539

Digital Object Identifier (DOI)

  • 10.1093/jn/131.8.2145

PubMed ID

  • 11481409

Additional Document Info

volume

  • 131

issue

  • 8