The biotin switch method for the detection of S-nitrosylated proteins. Academic Article uri icon

Overview

abstract

  • Many of the effects of nitric oxide are mediated by the direct modification of cysteine residues resulting in an adduct called a nitrosothiol. Here, we describe a novel method for detecting proteins that contain nitrosothiols. In this three-step procedure, nitrosylated cysteines are converted to biotinylated cysteines. Biotinylated proteins can then be detected by immunoblotting or can be purified by avidin-affinity chromatography. We include examples of the detection of S-nitrosylated proteins in brain lysates after in vitro S-nitrosylation, as well as the detection of endogenous S-nitrosothiols in selected neuronal proteins.

publication date

  • June 12, 2001

Research

keywords

  • Biotin
  • Biotinylation
  • Proteins
  • S-Nitrosothiols

Identity

Scopus Document Identifier

  • 0035849715

Digital Object Identifier (DOI)

  • 10.1126/stke.2001.86.pl1

PubMed ID

  • 11752655

Additional Document Info

volume

  • 2001

issue

  • 86