A cis-acting element that directs circular adeno-associated virus replication and packaging. Academic Article uri icon

Overview

abstract

  • A novel pathway of adeno-associated virus (AAV) replication marked by the assembly of circular monomer duplex intermediates (cAAV) has been recently discovered. In the present report we identify a single AD domain of the inverted terminal repeat as a minimal origin of cAAV replication. A small internal palindrome (BB'), necessary for optimal Rep-inverted terminal repeat interaction, does not contribute to the efficiency of cAAV replication, while the terminal resolution site is an essential cis-acting element. Furthermore, recombinant cAAV vectors that encompass only the AD domain replicate exclusively in a circular form and no detectable linear duplex replicative intermediates are generated, suggesting that both pathways of AAV replication are independent and can be separated. In addition, we show that cAAVs are efficient templates for encapsidation of single-stranded DNA genomes, an observation that assigns a biological role for these novel replication species. Together, these findings shed new light on the current model of AAV replication and packaging.

publication date

  • December 1, 2002

Research

keywords

  • Dependovirus
  • Terminal Repeat Sequences
  • Virus Assembly
  • Virus Replication

Identity

PubMed Central ID

  • PMC136660

Scopus Document Identifier

  • 0036893147

Digital Object Identifier (DOI)

  • 10.1128/jvi.76.24.12792-12802.2002

PubMed ID

  • 12438604

Additional Document Info

volume

  • 76

issue

  • 24