Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles. Academic Article uri icon

Overview

abstract

  • HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.

publication date

  • May 21, 2003

Research

keywords

  • Hepacivirus
  • Viral Envelope Proteins

Identity

PubMed Central ID

  • PMC165865

Scopus Document Identifier

  • 0038471344

Digital Object Identifier (DOI)

  • 10.1073/pnas.0832180100

PubMed ID

  • 12761383

Additional Document Info

volume

  • 100

issue

  • 12