Dynamic force spectroscopy of protein-DNA interactions by unzipping DNA. Academic Article uri icon

Overview

abstract

  • We demonstrate the first site-specific single-molecule characterization of the prominent activation barrier for the disruption of a protein-DNA binding complex. We achieved this new capability by combining dynamic force spectroscopy with unzipping force analysis of protein association and used the combination to investigate restriction enzyme binding to specific DNA sites. Analysis revealed lifetimes and interaction distances for three protein-DNA interactions. This new method is able to distinguish protein-DNA binding complexes on a site-specific, single-molecule basis.

publication date

  • July 10, 2003

Research

keywords

  • DNA
  • Deoxyribonucleases, Type II Site-Specific

Identity

Scopus Document Identifier

  • 0141499886

Digital Object Identifier (DOI)

  • 10.1103/PhysRevLett.91.028103

PubMed ID

  • 12906513

Additional Document Info

volume

  • 91

issue

  • 2