In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae. Academic Article uri icon

Overview

abstract

  • Vacuole inheritance is temporally coordinated with the cell cycle and is restricted spatially to an axis between the maternal vacuole and the bud. The new bud vacuole is founded by a stream of vacuole-derived membranous vesicles and tubules which are transported from the mother cell into the bud to form the daughter organelle. We now report in vitro formation of vacuole-derived tubules and vesicles. In semi-intact cells, formation of tubulovesicular structures requires ATP and the proteins encoded by VAC1 and VAC2, two genes which are required for vacuole inheritance in vivo. Isolation of vacuoles from cell lysates before in vitro incubation reveals that formation of tubulovesicular structures requires cytosol as well as ATP. After forming tubulovesicular structures, isolated vacuoles subsequently increase in size. Biochemical assays reveal that this increase results from vacuole to vacuole fusion, leading to mixing of organellar contents. Intervacuolar fusion is sensitive to the phosphatase inhibitors microcystin-LR and okadaic acid, suggesting that protein phosphorylation/dephosphorylation reactions play a role in this event.

publication date

  • December 1, 1992

Research

keywords

  • Cell Division
  • Extrachromosomal Inheritance
  • Saccharomyces cerevisiae
  • Vacuoles

Identity

PubMed Central ID

  • PMC2289757

Scopus Document Identifier

  • 0027093271

Digital Object Identifier (DOI)

  • 10.1083/jcb.119.6.1469

PubMed ID

  • 1334958

Additional Document Info

volume

  • 119

issue

  • 6