Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Academic Article uri icon

Overview

abstract

  • Nitric oxide (NO) conveys a variety of messages between cells, including signals for vasorelaxation, neurotransmission, and cytotoxicity. In some endothelial cells and neurons, a constitutive NO synthase is activated transiently by agonists that elevate intracellular calcium concentrations and promote the binding of calmodulin. In contrast, in macrophages, NO synthase activity appears slowly after exposure of the cells to cytokines and bacterial products, is sustained, and functions independently of calcium and calmodulin. A monospecific antibody was used to clone complementary DNA that encoded two isoforms of NO synthase from immunologically activated mouse macrophages. Liquid chromatography-mass spectrometry was used to confirm most of the amino acid sequence. Macrophage NO synthase differs extensively from cerebellar NO synthase. The macrophage enzyme is immunologically induced at the transcriptional level and closely resembles the enzyme in cytokine-treated tumor cells and inflammatory neutrophils.

publication date

  • April 10, 1992

Research

keywords

  • Amino Acid Oxidoreductases
  • Isoenzymes
  • Macrophages

Identity

Scopus Document Identifier

  • 0026569273

Digital Object Identifier (DOI)

  • 10.1126/science.1373522

PubMed ID

  • 1373522

Additional Document Info

volume

  • 256

issue

  • 5054