Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site. Academic Article uri icon

Overview

abstract

  • Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussis and Bordetella holmesii.

publication date

  • February 1, 2004

Research

keywords

  • AIDS-Related Opportunistic Infections
  • Bordetella pertussis
  • Rec A Recombinases
  • Whooping Cough

Identity

PubMed Central ID

  • PMC344463

Scopus Document Identifier

  • 1242291830

Digital Object Identifier (DOI)

  • 10.1128/JCM.42.2.847-849.2004

PubMed ID

  • 14766870

Additional Document Info

volume

  • 42

issue

  • 2