Notch 1 signaling regulates peripheral T cell activation. Academic Article uri icon

Overview

abstract

  • Notch signaling has been identified as an important regulator of leukocyte differentiation and thymic maturation. Less is known about the role of Notch signaling in regulating mature T cells. We examined the role of Notch 1 in regulating peripheral T cell activity in vitro and in vivo. Coligation of Notch 1 together with TCR and CD28 resulted in a dramatic inhibition of T cell activation, proliferation, and cytokine production. This effect was dependent on presenilin activity and induced the expression of HES-1, suggestive of Notch 1 signaling. Biochemical analysis demonstrated an inhibition of AKT and GSK3beta phosphorylation following Notch 1 engagement while other biochemical signals such as TCR and ERK phosphorylation remained intact. Similar effects were observed in vivo in an adoptive transfer model. Therefore, Notch 1 signaling may play an important role in regulating naive T cell activation and homeostasis.

publication date

  • April 1, 2004

Research

keywords

  • Lymphocyte Activation
  • Receptors, Cell Surface
  • Signal Transduction
  • T-Lymphocytes
  • Transcription Factors

Identity

Scopus Document Identifier

  • 1842785211

Digital Object Identifier (DOI)

  • 10.1016/s1074-7613(04)00081-0

PubMed ID

  • 15084270

Additional Document Info

volume

  • 20

issue

  • 4