Determining protein half-lives. Academic Article uri icon

Overview

abstract

  • Controlling the stability of cellular proteins is a fundamental way by which cells regulate growth, differentiation, survival, and development. Measuring the turnover rate of a protein is often the first step in assessing whether or not the function of a protein is regulated by proteolysis under specific physiological conditions. Over the years, procedures to determine the half-life of proteins in cultured eukaryotic cells have been well-established. This chapter describes in detail the two most frequently used methods, pulse-chase analysis and cycloheximide blocking, to determine a protein's half-life in yeast and cultured mammalian cells.

publication date

  • January 1, 2004

Research

keywords

  • Protein Processing, Post-Translational
  • Proteins

Identity

Scopus Document Identifier

  • 4644230021

Digital Object Identifier (DOI)

  • 10.1385/1-59259-816-1:067

PubMed ID

  • 15173609

Additional Document Info

volume

  • 284