Architecture of the native photosynthetic apparatus of Phaeospirillum molischianum. Academic Article uri icon

Overview

abstract

  • The ubiquity and importance of photosynthetic organisms in nature has made the molecular mechanisms of photosynthesis a widely studied subject at both structural and functional levels. A current challenge is to understand the supramolecular assembly of the proteins involved in photosynthesis in native membranes. We have used atomic force microscopy to study the architecture of the photosynthetic apparatus and analyze the structure of single molecules in chromatophores of Phaeospirillum molischianum. Core complexes are formed by the reaction center enclosed by an elliptical light harvesting complex 1. LH2 are octameric rings, assembled either with cores or in hexagonally packed LH2 antenna domains. The symmetry mismatch caused by octameric LH2 packing in a hexagonal lattice, that could be avoided in a square lattice, suggests lipophobic effects rather than specific inter-molecular interactions drive protein organization. The core and LH2 complexes are organized to form a supramolecular assembly reminiscent to that found in Rhodospirillum photometricum, and very different from that observed in Rhodobacter sphaeroides, Rb. blasticus, and Blastochloris viridis.

publication date

  • November 18, 2005

Research

keywords

  • Photosynthetic Reaction Center Complex Proteins
  • Rhodospirillum

Identity

Scopus Document Identifier

  • 28944431668

Digital Object Identifier (DOI)

  • 10.1016/j.jsb.2005.10.002

PubMed ID

  • 16330228

Additional Document Info

volume

  • 152

issue

  • 3