Human and mouse hemoglobin association with the transgenic mouse erythrocyte membrane. Academic Article uri icon

Overview

abstract

  • Transgenic mouse models of hemoglobinopathies unravel pathophysiological mechanisms; yet the validity of the red blood cell (RBC) model of human hemoglobin (hHb) enveloped by a mouse (m) membrane has been questioned. Isoelectric focusing of hHb and mHb from transgenic mRBC shows a greater association of mHb to the mouse membrane compared to normal hHbA, supporting a species-specific Hb-mRBC membrane interaction. Enhanced hmutant Hb (HbE, HbS and HbC)-mRBC membrane affinities correlates with enhanced membrane lipid peroxidation and parallel those reported in hRBC, lending support to transgenic mRBC as models of hemoglobinopathies. Species-specific Hb-membrane interaction may be overridden by Hb charge and conformational alterations.

publication date

  • July 17, 2006

Research

keywords

  • Erythrocyte Membrane
  • Hemoglobins

Identity

Scopus Document Identifier

  • 33746371697

Digital Object Identifier (DOI)

  • 10.1016/j.febslet.2006.07.021

PubMed ID

  • 16860794

Additional Document Info

volume

  • 580

issue

  • 18