An assembled complex IV maintains the stability and activity of complex I in mammalian mitochondria. Academic Article uri icon

Overview

abstract

  • In the mammalian mitochondrial electron transfer system, the majority of electrons enter at complex I, go through complexes III and IV, and are finally delivered to oxygen. Previously we generated several mouse cell lines with suppressed expression of the nuclearly encoded subunit 4 of complex IV. This led to a loss of assembly of complex IV and its defective function. Interestingly, we found that the level of assembled complex I and its activity were also significantly reduced, whereas levels and activity of complex III were normal or up-regulated. The structural and functional dependence of complex I on complex IV was verified using a human cell line carrying a nonsense mutation in the mitochondrially encoded complex IV subunit 1 gene. Our work documents that, although there is no direct electron transfer between them, an assembled complex IV helps to maintain complex I in mammalian cells.

publication date

  • April 23, 2007

Research

keywords

  • Electron Transport Complex I
  • Electron Transport Complex IV
  • Mitochondria

Identity

Scopus Document Identifier

  • 34547117624

Digital Object Identifier (DOI)

  • 10.1074/jbc.M701056200

PubMed ID

  • 17452320

Additional Document Info

volume

  • 282

issue

  • 24