G protein beta gamma subunit interaction with the dynein light-chain component Tctex-1 regulates neurite outgrowth. Academic Article uri icon

Overview

abstract

  • Tctex-1, a light-chain component of the cytoplasmic dynein motor complex, can function independently of dynein to regulate multiple steps in neuronal development. However, how dynein-associated and dynein-free pools of Tctex-1 are maintained in the cell is not known. Tctex-1 was recently identified as a Gbetagamma-binding protein and shown to be identical to the receptor-independent activator of G protein signaling AGS2. We propose a novel role for the interaction of Gbetagamma with Tctex-1 in neurite outgrowth. Ectopic expression of either Tctex-1 or Gbetagamma promotes neurite outgrowth whereas interfering with their function inhibits neuritogenesis. Using embryonic mouse brain extracts, we demonstrate an endogenous Gbetagamma-Tctex-1 complex and show that Gbetagamma co-segregates with dynein-free fractions of Tctex-1. Furthermore, Gbeta competes with the dynein intermediate chain for binding to Tctex-1, regulating assembly of Tctex-1 into the dynein motor complex. We propose that Tctex-1 is a novel effector of Gbetagamma, and that Gbetagamma-Tctex-1 complex plays a key role in the dynein-independent function of Tctex-1 in regulating neurite outgrowth in primary hippocampal neurons, most likely by modulating actin and microtubule dynamics.

publication date

  • May 10, 2007

Research

keywords

  • GTP-Binding Protein beta Subunits
  • GTP-Binding Protein gamma Subunits
  • Microtubule-Associated Proteins
  • Models, Molecular
  • Neurites
  • Nuclear Proteins

Identity

PubMed Central ID

  • PMC1888676

Scopus Document Identifier

  • 34250010871

Digital Object Identifier (DOI)

  • 10.1038/sj.emboj.7601716

PubMed ID

  • 17491591

Additional Document Info

volume

  • 26

issue

  • 11