PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Academic Article uri icon

Overview

abstract

  • The assembly of the spliceosome is an ATP-dependent process. The splicing factor PRP16 contains variations of several motifs that define the eIF-4A-like ATP-dependent RNA helicase family. The protein has now been purified and shown to exhibit RNA-dependent ATPase activity. PRP16 is required specifically for the second catalytic step of the splicing reaction in vitro. This function requires ATP binding and/or hydrolysis, which appears to be concomitant with release of the protein from the spliceosome. PRP16 may be the prototype for a set of splicing factors which use ATP to drive a cycle of conformational changes.

publication date

  • February 7, 1991

Research

keywords

  • Adenosine Triphosphatases
  • RNA Splicing

Identity

Scopus Document Identifier

  • 0026019713

Digital Object Identifier (DOI)

  • 10.1038/349494a0

PubMed ID

  • 1825134

Additional Document Info

volume

  • 349

issue

  • 6309