Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts. Academic Article uri icon

Overview

abstract

  • Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.

publication date

  • October 16, 2009

Research

keywords

  • Gene Expression Profiling
  • Mutation
  • Neoplasms
  • Oncogene Proteins, Fusion
  • Sequence Analysis, DNA

Identity

PubMed Central ID

  • PMC2784330

Scopus Document Identifier

  • 75349100601

Digital Object Identifier (DOI)

  • 10.1101/gr.GR-1871R

PubMed ID

  • 19835606

Additional Document Info

volume

  • 10

issue

  • 10