CCM3 signaling through sterile 20-like kinases plays an essential role during zebrafish cardiovascular development and cerebral cavernous malformations. Academic Article uri icon

Overview

abstract

  • Cerebral cavernous malformation is a common human vascular disease that arises due to loss-of-function mutations in genes encoding three intracellular adaptor proteins, cerebral cavernous malformations 1 protein (CCM1), CCM2, and CCM3. CCM1, CCM2, and CCM3 interact biochemically in a pathway required in endothelial cells during cardiovascular development in mice and zebrafish. The downstream effectors by which this signaling pathway regulates endothelial function have not yet been identified. Here we have shown in zebrafish that expression of mutant ccm3 proteins (ccm3Delta) known to cause cerebral cavernous malformation in humans confers cardiovascular phenotypes identical to those associated with loss of ccm1 and ccm2. CCM3Delta proteins interacted with CCM1 and CCM2, but not with other proteins known to bind wild-type CCM3, serine/threonine protein kinase MST4 (MST4), sterile 20-like serine/threonine kinase 24 (STK24), and STK25, all of which have poorly defined biological functions. Cardiovascular phenotypes characteristic of CCM deficiency arose due to stk deficiency and combined low-level deficiency of stks and ccm3 in zebrafish embryos. In cultured human endothelial cells, CCM3 and STK25 regulated barrier function in a manner similar to CCM2, and STKs negatively regulated Rho by directly activating moesin. These studies identify STKs as essential downstream effectors of CCM signaling in development and disease that may regulate both endothelial and epithelial cell junctions.

publication date

  • July 1, 2010

Research

keywords

  • Apoptosis Regulatory Proteins
  • Cardiovascular System
  • Hemangioma, Cavernous, Central Nervous System
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Protein Serine-Threonine Kinases
  • Proto-Oncogene Proteins
  • Signal Transduction
  • Zebrafish Proteins

Identity

PubMed Central ID

  • PMC2912181

Scopus Document Identifier

  • 77955287380

Digital Object Identifier (DOI)

  • 10.1002/gene.10248

PubMed ID

  • 20592472

Additional Document Info

volume

  • 120

issue

  • 8