Expression of collagen biosynthetic activities in lymphocytic cells. Academic Article uri icon

Overview

abstract

  • Immunoglobulin-producing Merwin plasma cells, MPC-11, have been found to contain proplyl hydroxylase (prolyl-glycyl-peptide,2-oxoglutarate:oxygen oxidoreductase; EC 1.14.11.2) activity and its crossreacting protein, as well as hydroxyproline and a collagenous protein that could not be classified as type I, II, or III collagen. Friend leukemic cells, on the other hand, contained only prolyl hydroxylase. Thymus-derived (T) lymphocytes and bone-marrow-derived (B) lymphocytes freshly isolated from BALB/c mice expressed low but significant prolyl hydroxylase activity. Upon stimulation with phytohemagglutinin, the enzyme activity in T cells increased 22- to 29-fold. Crossreacting protein was also increased and appeared more stable than the prolyl hydroxylase. The effect of lipopolysaccharide stimulation on B cells uas similar but not as pronounced. Thus, even when not accompanied by other collagen biosynthetic activities, prolyl hydroxylase is present in all cells of hematologic origin.

publication date

  • March 1, 1978

Research

keywords

  • Collagen
  • Lymphocytes
  • Procollagen-Proline Dioxygenase

Identity

PubMed Central ID

  • PMC411475

Scopus Document Identifier

  • 0018097152

Digital Object Identifier (DOI)

  • 10.1073/pnas.75.3.1379

PubMed ID

  • 206896

Additional Document Info

volume

  • 75

issue

  • 3