Expression and localization of claudins-3 and -12 in transformed human brain endothelium. Academic Article uri icon

Overview

abstract

  • BACKGROUND: The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. FINDINGS: hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture. CONCLUSIONS: These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro.

publication date

  • February 29, 2012

Identity

PubMed Central ID

  • PMC3305566

Scopus Document Identifier

  • 34547401535

Digital Object Identifier (DOI)

  • 10.1111/j.1471-4159.2007.04563.x

PubMed ID

  • 22373538

Additional Document Info

volume

  • 9