Loss of acinar cell IKKα triggers spontaneous pancreatitis in mice. Academic Article uri icon

Overview

abstract

  • Chronic pancreatitis is an inflammatory disease that causes progressive destruction of pancreatic acinar cells and, ultimately, loss of pancreatic function. We investigated the role of IκB kinase α (IKKα) in pancreatic homeostasis. Pancreas-specific ablation of IKKα (Ikkα(Δpan)) caused spontaneous and progressive acinar cell vacuolization and death, interstitial fibrosis, inflammation, and circulatory release of pancreatic enzymes, clinical signs resembling those of human chronic pancreatitis. Loss of pancreatic IKKα causes defective autophagic protein degradation, leading to accumulation of p62-mediated protein aggregates and enhanced oxidative and ER stress in acinar cells, but none of these effects is related to NF-κB. Pancreas-specific p62 ablation prevented ER and oxidative stresses and attenuated pancreatitis in Ikkα(Δpan) mice, suggesting that cellular stress induced by p62 aggregates promotes development of pancreatitis. Importantly, downregulation of IKKα and accumulation of p62 aggregates were also observed in chronic human pancreatitis. Our studies demonstrate that IKKα, which may control autophagic protein degradation through its interaction with ATG16L2, plays a critical role in maintaining pancreatic acinar cell homeostasis, whose dysregulation promotes pancreatitis through p62 aggregate accumulation.

publication date

  • April 8, 2013

Research

keywords

  • Acinar Cells
  • Gene Expression Regulation, Enzymologic
  • I-kappa B Kinase
  • Pancreatitis

Identity

PubMed Central ID

  • PMC3635720

Scopus Document Identifier

  • 84876789628

Digital Object Identifier (DOI)

  • 10.1172/JCI64209

PubMed ID

  • 23563314

Additional Document Info

volume

  • 123

issue

  • 5