23Na-nuclear magnetic resonance investigation of gramicidin-induced ion transport through membranes under equilibrium conditions. Academic Article uri icon

Overview

abstract

  • A technique for investigating the gramicidin-facilitated transport of Na+ ions across lipid bilayers of large unilamellar vesicles under the condition of ionic equilibrium has been developed using a combination of heat incubation of the gramicidin with the vesicles and 23Na-nuclear magnetic resonance (NMR) spectroscopy. Isolation of the two 23Na-NMR signals from the intra- and extravesicular Na+ with the shift reagent, dysprosium (III) tripolyphosphate, allows the equilibrium flux of Na+ through the gramicidin channels to be detected and treated as a two-site exchange process. This study indicates that the transport of Na+ through gramicidin channels is second order with respect to the gramicidin concentration.

publication date

  • February 1, 1988

Research

keywords

  • Gramicidin
  • Ion Channels
  • Lipid Bilayers
  • Phosphatidylcholines
  • Phosphatidylglycerols

Identity

PubMed Central ID

  • PMC1330135

Scopus Document Identifier

  • 0023818842

Digital Object Identifier (DOI)

  • 10.1016/S0006-3495(88)83076-5

PubMed ID

  • 2449917

Additional Document Info

volume

  • 53

issue

  • 2