Regulation of antibody secretion by hybridoma cells. II. Mechanism of idiotype-induced suppression of antibody secretion by hybridoma cells. Academic Article uri icon

Overview

abstract

  • We have previously reported that antibody secretion by B.22 hybridoma cells can be suppressed in an MHC-restricted manner, by idiotype-specific T cells. It was shown that T cells of both helper and suppressor phenotypes are involved, and that the suppression is mediated by soluble factors. In the present paper, we have characterized the effects of T-cell-mediated suppression at the level of B.22 antibody mRNA expression and stability. Nuclear run-on analysis comparing suppressed and control B.22 cells indicates no change in the transcription rates of heavy and light chains. Northern blot analysis demonstrates that steady-state levels of heavy and light chain mRNAs are also unchanged. Thus, the suppression of antibody secretion by B.22 cells probably occurs at the levels of translation or secretion.

publication date

  • October 15, 1989

Research

keywords

  • Antibodies, Monoclonal
  • Hybridomas
  • Immunoglobulin Idiotypes
  • T-Lymphocytes, Helper-Inducer

Identity

Scopus Document Identifier

  • 0024434459

Digital Object Identifier (DOI)

  • 10.1016/0008-8749(89)90288-8

PubMed ID

  • 2529040

Additional Document Info

volume

  • 123

issue

  • 2