Regulated Expression Systems for Mycobacteria and Their Applications. Academic Article uri icon

Overview

abstract

  • For bacterial model organisms like Escherichia coli and Bacillus subtilis genetic tools to experimentally manipulate the activity of individual genes existed for decades. But for genetically less tractable yet medically important bacteria such as M. tuberculosis such tools have rarely been available. More recently several groups developed genetic switches that function efficiently in M. tuberculosis and other mycobacteria. Together these systems utilize six different transcription factors, eight different regulated promoters, and three different regulatory principles. Here we describe their design features, review their main applications, and discuss advantages and disadvantages of regulating transcription, translation, or protein stability for controlling gene activities in bacteria.

publication date

  • January 1, 2014

Identity

PubMed Central ID

  • PMC4254785

Digital Object Identifier (DOI)

  • 10.1128/microbiolspec.MGM2-0018-2013

PubMed ID

  • 25485177

Additional Document Info

volume

  • 2

issue

  • 1