A dynein independent role of Tctex-1 at the kinetochore. Academic Article uri icon

Overview

abstract

  • Dynein light chains are accessory subunits of the cytoplasmic dynein complex, a minus-end directed microtubule motor. Here, we demonstrate that the dynein light chain Tctex-1 associates with unattached kinetochores and is essential for accurate chromosome segregation. Tctex-1 knockdown in cells does not affect the localization and function of dynein at the kinetochore, but produces a prolonged mitotic arrest with a few misaligned chromosomes, which are subsequently missegregated during anaphase. This function is independent of Tctex-1's association with dynein. The kinetochore localization of Tctex-1 is independent of the ZW10-dynein pathway, but requires the Ndc80 complex. Thus, our findings reveal a dynein independent role of Tctex-1 at the kinetochore to enhance the stability of kinetochore-microtubule attachment.

publication date

  • January 1, 2015

Research

keywords

  • Dyneins
  • Kinetochores
  • Microtubules

Identity

PubMed Central ID

  • PMC4614829

Scopus Document Identifier

  • 84983494273

Digital Object Identifier (DOI)

  • 10.1073/pnas.0908959107

PubMed ID

  • 25928583

Additional Document Info

volume

  • 14

issue

  • 9