Evaluation of Remel Spectra CRE Agar for Detection of Carbapenem-Resistant Bacteria from Rectal Swabs Obtained from Residents of a Long-Term-Care Facility. Academic Article uri icon

Overview

abstract

  • We compared the Remel Spectra CRE agar plate to CDC standard methodology for the isolation of carbapenem-resistant Enterobacteriaceae (CRE) from 300 rectal swab specimens obtained from patients residing in a long-term-care facility (LTCF). Multiplex PCR experiments were performed on isolates to identify specific Klebsiella pneumoniae carbapenemases (KPC) and additional β-lactamases. Of the 300 patients, 72 (24%) harbored CRE and were PCR positive for KPC enzymes. The Remel Spectra CRE plates detected KPC-type CRE in isolates from 70 of 72 patients (97.2%), while the CDC method detected CRE in 56 of 72 (77.8%). CRE identification results were available in 18 h compared to 36 h for the CDC method. Remel Spectra CRE agar plates can provide useful means for a fast and reliable method for detecting KPC-type CRE and for accelerated institution of appropriate infection control precautions.

publication date

  • June 17, 2015

Research

keywords

  • Anti-Bacterial Agents
  • Carbapenems
  • Culture Media
  • Enterobacteriaceae
  • Enterobacteriaceae Infections
  • beta-Lactam Resistance

Identity

PubMed Central ID

  • PMC4540922

Scopus Document Identifier

  • 84940055904

Digital Object Identifier (DOI)

  • 10.1111/j.1532-5415.2011.03881.x

PubMed ID

  • 26085613

Additional Document Info

volume

  • 53

issue

  • 9