Gene expression profiling signatures for the diagnosis and prevention of oral cavity carcinogenesis-genome-wide analysis using RNA-seq technology. Academic Article uri icon

Overview

abstract

  • We compared the changes in global gene expression between an early stage (the termination of the carcinogen treatment and prior to the appearance of frank tumors) and a late stage (frank squamous cell carcinoma (SCC)) of tongue carcinogenesis induced by the carcinogen 4-nitroquinoline 1-oxide (4-NQO) in a mouse model of human oral cavity and esophageal squamous cell carcinoma. Gene ontology and pathway analyses show that increases in "cell cycle progression" and "degradation of basement membrane and ECM pathways" are early events during SCC carcinogenesis and that changes in these pathways are even greater in the actual tumors. Myc, NFκB complex (NFKB1/RELA), and FOS transcription networks are the major transcriptional networks induced in early stage tongue carcinogenesis. Decreases in metabolism pathways, such as in "tricarboxylic acid cycle" and "oxidative phosphorylation", occurred only in the squamous cell carcinomas and not in the early stages of carcinogenesis. We detected increases in ALDH1A3, PTGS2, and KRT1 transcripts in both the early and late stages of carcinogenesis. The identification of the transcripts and pathways that change at an early stage of carcinogenesis provides potentially useful information for early diagnosis and for prevention strategies for human tongue squamous cell carcinomas.

publication date

  • September 15, 2015

Research

keywords

  • Carcinogenesis
  • Carcinoma, Squamous Cell
  • Esophageal Neoplasms
  • Gene Expression Regulation, Neoplastic
  • Mouth Neoplasms
  • Tongue Neoplasms

Identity

PubMed Central ID

  • PMC4695195

Scopus Document Identifier

  • 84943394029

Digital Object Identifier (DOI)

  • 10.18632/oncotarget.4420

PubMed ID

  • 26110572

Additional Document Info

volume

  • 6

issue

  • 27