Promoter-like epigenetic signatures in exons displaying cell type-specific splicing. Academic Article uri icon

Overview

abstract

  • BACKGROUND: Pre-mRNA splicing occurs mainly co-transcriptionally, and both nucleosome density and histone modifications have been proposed to play a role in splice site recognition and regulation. However, the extent and mechanisms behind this interplay remain poorly understood. RESULTS: We use transcriptomic and epigenomic data generated by the ENCODE project to investigate the association between chromatin structure and alternative splicing. We find a strong and significant positive association between H3K9ac, H3K27ac, H3K4me3, epigenetic marks characteristic of active promoters, and exon inclusion in a small but well-defined class of exons, representing approximately 4 % of all regulated exons. These exons are systematically maintained at comparatively low levels of inclusion across cell types, but their inclusion is significantly enhanced in particular cell types when in physical proximity to active promoters. CONCLUSION: Histone modifications and other chromatin features that activate transcription can be co-opted to participate in the regulation of the splicing of exons that are in physical proximity to promoter regions.

publication date

  • October 23, 2015

Research

keywords

  • Alternative Splicing
  • Epigenesis, Genetic
  • Exons
  • Promoter Regions, Genetic

Identity

PubMed Central ID

  • PMC4619081

Scopus Document Identifier

  • 84944879577

Digital Object Identifier (DOI)

  • 10.1093/nar/gkp335

PubMed ID

  • 26498677

Additional Document Info

volume

  • 16