Transfection of Streptococcus pneumoniae with bacteriophage DNA. Academic Article uri icon

Overview

abstract

  • It was possible to transfect Streptococcus pneumoniae with DNA obtained from a newly isolated bacteriophage, diplophage-4 (Dp-4). Optimal frequency of transfection (0.9%) required the use of a nuclease-defective mutant; with wild-type bacteria, the transfection frequency was about 100-fold lower. Transfection requires physiological conditions that appear to be similar to the competent state needed for genetic transformation (A. Tomasz, J. Bacteriol. 91:1050--1061, 1966).

publication date

  • May 1, 1978

Research

keywords

  • Bacteriophages
  • Streptococcus pneumoniae
  • Transfection

Identity

PubMed Central ID

  • PMC354058

Scopus Document Identifier

  • 0018139822

Digital Object Identifier (DOI)

  • 10.1128/JVI.26.2.221-225.1978

PubMed ID

  • 26809

Additional Document Info

volume

  • 26

issue

  • 2