A nucleoprotein complex mediates the integration of retroviral DNA. Academic Article uri icon

Overview

abstract

  • The integration of viral DNA into the host genome is an essential step in the retrovirus life cycle. To understand this process better, we have examined the native state of viral DNA in cells acutely infected by murine leukemia virus (MLV), using both a physical assay for viral DNA and a functional assay for integration activity (Brown et al. 1987). The viral DNA and integration activity copurify during velocity sedimentation, gel filtration, and density equilibrium centrifugation, indicating that viral DNA is in a large (approximately 160S) nucleoprotein complex that includes all functions required for integration activity in vitro. Analysis by immunoprecipitation shows that the viral capsid protein is part of the active nucleoprotein complex, but recognition of the complex by only a subset of anti-capsid sera implies that the protein is constrained conformationally. The viral DNA within this structure is accessible to nucleases; the effects of nucleases on the integrity of the complex suggest that the integration-competent particle is derived from and similar to the core of extracellular virions.

publication date

  • April 1, 1989

Research

keywords

  • DNA, Viral
  • Nucleoproteins
  • Recombination, Genetic
  • Retroviridae

Identity

Scopus Document Identifier

  • 0024654338

Digital Object Identifier (DOI)

  • 10.1101/gad.3.4.469

PubMed ID

  • 2721960

Additional Document Info

volume

  • 3

issue

  • 4