Immunofluorescent Staining of Mouse Intestinal Stem Cells. Academic Article uri icon

Overview

abstract

  • Immunofluorescent staining of organoids can be performed to visualize molecular markers of cell behavior. For example, cell proliferation marked by incorporation of nucleotide (EdU), or to observe markers of intestinal differentiation including paneth cells, goblet cells, or enterocytes (see Figure 1). In this protocol we detail a method to fix, permeabilize, stain and mount intestinal organoids for analysis by immunofluorescent confocal microscopy.

publication date

  • February 20, 2016

Identity

PubMed Central ID

  • PMC4996654

Digital Object Identifier (DOI)

  • 10.21769/bioprotoc.1732

PubMed ID

  • 27570798

Additional Document Info

volume

  • 6

issue

  • 4