Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. Academic Article uri icon

Overview

abstract

  • In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and multilineage differentiation potential over time. This provides investigators a tool to culture wild type or transformed intestinal epithelium, and a robust assay for stem cell tissue homeostasis in vitro.

publication date

  • February 20, 2016

Identity

PubMed Central ID

  • PMC4996657

Digital Object Identifier (DOI)

  • 10.21769/bioprotoc.1733

PubMed ID

  • 27570799

Additional Document Info

volume

  • 6

issue

  • 4