Multicenter Performance Assessment of Carba NP Test. Academic Article uri icon

Overview

abstract

  • Eighty Gram-negative bacilli (54 Enterobacteriaceae and 26 nonfermenting Gram-negative bacilli) obtained from multiple institutions in the United States were distributed in a blinded manner to seven testing laboratories to compare their performance of a test for detection of carbapenemase production, the Carba NP test. The Carba NP test was performed by all laboratories, following the Clinical and Laboratory Standards Institute (CLSI) procedure. Site-versus-site comparisons demonstrated a high level of consistency for the Carba NP assay, with just 3/21 site comparisons yielding a difference in sensitivity (P < 0.05). Previously described limitations with blaOXA-48-like carbapenemases and blaOXA carbapenemases associated with Acinetobacter baumannii were noted. Based on these data, we demonstrate that the Carba NP test, when implemented with the standardized CLSI methodology, provides reproducible results across multiple sites for detection of carbapenemases.

publication date

  • April 12, 2017

Research

keywords

  • Bacterial Proteins
  • Bacteriological Techniques
  • Enterobacteriaceae
  • beta-Lactamases

Identity

PubMed Central ID

  • PMC5442553

Scopus Document Identifier

  • 85019620148

Digital Object Identifier (DOI)

  • 10.1016/j.jclinepi.2014.03.012

PubMed ID

  • 28404676

Additional Document Info

volume

  • 55

issue

  • 6