Prospective isolation of NKX2-1-expressing human lung progenitors derived from pluripotent stem cells. Academic Article uri icon

Overview

abstract

  • It has been postulated that during human fetal development, all cells of the lung epithelium derive from embryonic, endodermal, NK2 homeobox 1-expressing (NKX2-1+) precursor cells. However, this hypothesis has not been formally tested owing to an inability to purify or track these progenitors for detailed characterization. Here we have engineered and developmentally differentiated NKX2-1GFP reporter pluripotent stem cells (PSCs) in vitro to generate and isolate human primordial lung progenitors that express NKX2-1 but are initially devoid of differentiated lung lineage markers. After sorting to purity, these primordial lung progenitors exhibited lung epithelial maturation. In the absence of mesenchymal coculture support, this NKX2-1+ population was able to generate epithelial-only spheroids in defined 3D cultures. Alternatively, when recombined with fetal mouse lung mesenchyme, the cells recapitulated epithelial-mesenchymal developing lung interactions. We imaged these progenitors in real time and performed time-series global transcriptomic profiling and single-cell RNA sequencing as they moved through the earliest moments of lung lineage specification. The profiles indicated that evolutionarily conserved, stage-dependent gene signatures of early lung development are expressed in primordial human lung progenitors and revealed a CD47hiCD26lo cell surface phenotype that allows their prospective isolation from untargeted, patient-specific PSCs for further in vitro differentiation and future applications in regenerative medicine.

publication date

  • May 2, 2017

Research

keywords

  • Induced Pluripotent Stem Cells
  • Nuclear Proteins
  • Transcription Factors

Identity

PubMed Central ID

  • PMC5451263

Scopus Document Identifier

  • 85020209962

Digital Object Identifier (DOI)

  • 10.1016/S0012-1606(03)00117-9

PubMed ID

  • 28463226

Additional Document Info

volume

  • 127

issue

  • 6