T-cell receptor gene rearrangements as markers of lineage and clonality in T-cell neoplasms. Academic Article uri icon

Overview

abstract

  • Ig gene rearrangements represent markers of lineage, clonality, and differentiation of B cells, allowing a molecular diagnosis and immunogenotypic classification of B-cell neoplasms. We sought to apply a similar approach to the study of T-cell populations by analyzing rearrangements of the T-cell receptor beta-chain (T beta) gene. Our analysis, by Southern blotting hybridization using T beta-specific probes of DNAs from polyclonal T cells and from 12 T-cell tumors, indicates that T beta gene rearrangement patterns can be used as markers of (i) lineage, allowing the identification of polyclonal T-cell populations, and (ii) clonality, allowing the detection of monoclonal T-cell tumors. In addition, our data indicate that T beta gene rearrangements represent early and general markers of T-cell differentiation since they are detectable in histologically different tumors at all stages of T-cell development. The ability to determine lineage, clonality, and stage of differentiation has significant implications for future experimental and clinical studies on normal and neoplastic T cells.

publication date

  • May 1, 1985

Research

keywords

  • DNA, Neoplasm
  • Leukemia
  • Lymphoma
  • Receptors, Antigen, T-Cell
  • T-Lymphocytes

Identity

PubMed Central ID

  • PMC397795

Scopus Document Identifier

  • 3342989105

Digital Object Identifier (DOI)

  • 10.1073/pnas.82.10.3460

PubMed ID

  • 2987928

Additional Document Info

volume

  • 82

issue

  • 10